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KMID : 0380020060210030194
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 3 p.194 ~ p.198
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Extraction of β-carotene from Ascidian Tunic (Halocynthia roretzi ) using Supercritical Carbon Dioxide and Co-solvent
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Kang In-Sook
Youn Hyun-Seok
Park Ji-Yeon
Chun Byung-Soo
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Abstract
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Dried raw Ascidians (Halocynthia roretzi) shells harvested from fish farms in southern coast area in Korea were used to extract β-carotene using supercritical carbon dioxide (SCO2) and with ethanol as a co-solvent at the range of temperatures and pressures, from 25 to 65℃ and 100 to 350 bar respectively. The size of the dried Ascidians shells was around 850 μm. The system used this study was a semi-batch flow type high pressure unit. The efficiency of β-carotene extraction using SCO2 with and without co-solvent, ethanol, influenced to pressure and temperature changes. The highest solubility of β-carotene in SCO2 was 1.35 mg/g for β-carotene at 35℃ and 350 bar. With addition of 2 (v/v%) ethanol the recovery of β-carotene was 93%. As a result of using n-hexane and methanol for rinse, at 35℃ and 350 bar the amount of β-carotene by methanol rinse was 5 times higher than that of n-hexane rinse.
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KEYWORD
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Ascidians (Halocynthia roretzi), β-carotene, co-solvent, supercritical carbon dioxide
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